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ISC

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ISC Therapy

IMMUNE SIGNALING CELLS

BLOOMMED’s simplistic, minimally invasive method of immuno-regulating cellular therapy is at the forefront of Regenerative Medicine.

Immune Signaling Cells (ISC) therapy is an autologous immuno-regulating cellular therapy aimed at regulating the immune system which is responsible for the healing process. In a healthy individual’s immune system surveillance can detect cellular irregularities, damages or any invading pathogen which will trigger the body’s defense and healing processes such as inflammation, regeneration, and remodeling to restore function.

However, factors such as aging, lifestyle, stress, exposure to pollution or chemicals can interrupt cellular signaling which causes our immune system to malfunction. A compromised immune system caused by poor cellular communication can translate to the inability to trigger the healing process, prolonged inflammation (M1/M2 macrophages) where the body cannot progress to the repair stage or insufficient stem-like cells (stem cells, fibrocytes, and fibroblasts) to repair and regenerate the damaged cells or tissues.

Disease Management and Prevention

Disease is usually caused by chronic inflammation or prolong faulty cell signals, from the most basic cellular level, then progressively worsen and degenerate to a larger scale that impact the normal function of tissue and organ in our body. Therefore, it is vital to detect the cell errors in our body and enhance the repairment in order to efficiently prevent or stop the disease progression. ISC (Immune Signaling Cells) basically detects and amplify the optimal cell signal and then trigger the body’s self-healing process, while also give support to the tissue repair and regeneration simultaneously.

What is Immune Signaling Cells (ISC)?

Immune Signaling Cells (ISC) is an activated monocyte carrying and secreting a large amount of cell signaling protein or molecules. Monocyte and macrophage are part of the body’s innate immune system and the first responder to tissue injury by detecting the degeneration naturally.

Upon ex-vivo activation, we stimulate the monocyte into macrophage and brings out the heterogeneous of macrophage to express a great diversity of signaling proteins, which recruit and activate all the cells to work together throughout the healing process, from phagocytosis (expel damage cell, dead cell or pathogen), angiogenesis (boost the blood vessel to enhance the molecular and cell delivery), cell migration (transmit the cell to injured site), to induce the mitogenesis (cell division/ cell proliferation) of fibroblast and progenitor cell, which are responsible for tissue regeneration, while also enhance the extracellular matrix secretion to support the tissue repair, and finally to recruit or activate dormant stem cell for further regeneration.

Finally, patients no longer have to endure painful and invasive procedures to undergo cell treatment. With our unique Immune Signaling Cells (ISC), and a minimalistic methodology, we can deliver results swiftly and safely.

ISC MECHANISM & BENEFITS

ISC CULTURE PROCESS

Immune Signaling Cells
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01 BLOOD COLLECTION

Approximately 250 mL of blood will be collected from the patient for the ISC conversion.
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02 CELL SELECTION

Mononuclear Cells
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03 NUTRIENT MEDIUM

Human Grade, Quality & Safety assurance
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04 CELL CULTURE

Unique microenvironment and fastest turn-around time (96 hours)
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05 ISC RELEASE

Consist of Non-classical monocyte, Fibrocyte, Cytokine and Growth Factor. Sterility test approved.
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06 IV REINFUSION

Depending on your health requirements, we will tailor the best course of treatment for you.
ABOUT

THE ISC COMPOSITION

Our patented microenvironment for culturing Immune Signaling Cells (ISC) will favor the cell differentiation of monocyte into non-classical monocyte, fibrocyte and secrete enriched cytokines and growth factors from autologous mononuclear cells. These signaling cells are the foundation required to regulate the immune system to function as intended.

ISC culture medium was designed for monocyte activation and transition/conversion favour.  All of our culture medium are human graded and GMP manufactured.

01 ISC provides the key for M2 macrophage activation and enhances the anti-inflammatory cytokines secretion, such as IL-10 and TGF-β to suppress the inflammation and facilitate the process of tissue repair.

02 ISC assists the trauma environment shift from inflammatory to anti-inflammatory, which changes the chemical composition of the wound to a cell-regenerate-favored niche with abundant growth factors, such as VEGF, IGF-1 and PDGF, and sufficient blood flow.

03ISC also provides fibroblast-like CD34+ fibrocytes, which can rapidly enter the sites of injury, enhance the synthesis of extracellular matrix (ECM) and activate the resident stem cells for the cell regeneration and tissue repair.

Non-Classical Monocytes

Monocyte and macrophage play a critical role in the inflammation regulation and tissue repair and the M1/M2 macrophages modulate the phases transition in the wound healing process.

As the phases change, the macrophage population transitions from predominantly pro-inflammatory M1 macrophage to anti-inflammatory M2 macrophage, which dominate the later wound healing process as a key mediator for cell proliferation and tissue remodelling.

The circulating non-classical monocytes will further transition into M2 macrophage for inflammation control, which is impaired in the patients with chronic disease, autoimmune disease and chronic would.

By increasing the population of M2 macrophages, damaged tissue will shift from pro-inflammatory phase into anti-inflammatory phase, and as phagocytosis reaction declines, new cells are able to form and structure without being destroyed.

Fibrocytes

Circulating fibrocytes expresses stem-cell-like characteristic, once they are recruited to the site of injury, and it will further differentiate into fibroblasts. Fibroblasts are known as tissue resident mesenchymal cell and phenotypically undistinguished with the mesenchymal stromal cell (MSC) (Soundararajan M. et al., 2018). When the healing process reaches the repair stage M2 macrophage will recruit fibrocytes and activates the surrounding fibroblasts to undergo proliferation and start repairing the damaged tissue/cells. The regeneration process commences by the production of extracellular matrix which triggers tissue granulation and tissue framework stabilization to allow the new formation of connective tissues.

Cytokines & Growth Factors

Cytokines and growth factors are the fundamental communicating channel between cells. When cellular signaling is interrupted or desynchronized, the immune system will not be able to identify or respond to irregularity, giving rise to loss of homeostasis and leading to other complications. The production of ISC will produce an enriched number of cytokines and growth factors based on the pre-existing inflammation signals in the patient’s peripheral blood. When the cytokines and growth factors are reintroduced into the patient’s body, it will elevate the signals (as a stimulus) to the immune system to initiate the healing process. Growth factors can also accelerate the rate of repair and regeneration.

HOW OUR BODY

Chronic wound healing is a complex and dynamic process regulated by the immune system. The immune system not only fights off bacteria but also regulates the entire healing process, which can be divided into 4 distinct phases – TRIGGER, INFLAMMATION, REPAIR AND RESTORE.

WOUND

  • Platelets gather to form clot and stop the bleeding
  • PLT release cytokine SDF-1 to attract neutrophils and monocytes migrating to injured site by interacting with the neutrophils and monocytes surface marker CXCR-4

INFLAMMATION

  • Neutrophils come to sanitize the wound by producing high level ROS (reactive oxygen species)
  • Classical monocytes (CD14+CD16-) differentiate into M1 macrophages for neutrophils and pathogens phagocytosis by releasing IL-1, IL-6 and TNF-α

PROLIFERATION

  • Both circulating non-classical monocytes (CD14+CD16+) and resident M1 Macrophage transition into M2-like macrophage, which release anti-inflammation cytokine and growth factor, such as PDGF, IGF-1, VEGF, TGF-β and TIMP1 aiding in cellular proliferation, granulation tissue(extracellular matrix, ECM formation) and angiogenesis.

REMODELLING

  • Besides promoting the  angiogenesis, macrophage also help the position, stabilization and fusion of neovascular (newly forming blood vessels)
  • M2 macrophage assist the apoptosis of myofibroblast by secreting MMP-2 and MMP-9 to minimize the fibrosis (scar tissue) and supress the T-cell proliferation to return and restore homeostasis.

Key Factors for the body to effectively heal, repair and regenerate to restore back to its optimal function:

Immune Cell Activation

The ability of the immune system to detect damaged cells or tissues that require repair or replacement.

Optimal Cellular Signaling

To transition the communication language between cell and cell, which directs the immune response to attack harmful stimulation and regulates the healing process to repair the damage. Cellular signalling is the key to maintain a balanced and optimal body condition.

Stem Cells Activation

Stem Cells Activation (MSC, fibroblast or fibrocyte) – the building material for damage repairment and tissue regeneration.

Why is BloomMed’s ISC Needed?

This is where BLOOMMED’s innovative solution comes in with its revolutionary ISC therapy consisting of anti-inflammatory monocytes which are the key to address the inability of pro-inflammatory macrophage to turn into the anti-inflammatory macrophage. This step is crucial to resume the healing process for the body. ISC also produces numerous cytokines and growth factors that will benefit the local cells and enhance the coordinated healing and repair process to efficiently prevent the disease or wound progression. Immune Signaling Cells (ISC) basically detects and amplify the optimal cell signal and then trigger the body’s self-healing process, while also reinforcing the tissue repair and regeneration simultaneously.

Our Unique Difference

What Sets ISC Therapy Apart From The Rest?

Although stem cell treatment is hugely marketed and vastly popular, very little is known on its exact therapeutic mechanism. It is a given that it will only benefit the patient if the healing process can progress into the repair stage before the stem cells could be of any use. However for patients with chronic inflammation or lacking the stimulus to start the healing process within the immune system, injecting stem cells without understanding the entire process will show very minimal results. The stem cells would not be able to perform its job if the healing process is inhibited.

ISC therapy is different because we understand the healing process entirely and the therapy is specifically designed to ensure firstly the modulation of the immune system would provide the right cellular signaling for the progress to the repair stage is initiated and maximized. The ISC solution is much more effective because compared to conventional therapies, it targets the source of the issue. This, in turn, would optimize the efficacy of the stem cells.

BASIC PACKAGE

ISCs are cultured safely in a closed loop system

  • No hazardous chemicals or animal bovine serum that could cause rejection if reinfused into the body.
  • Cultured in closed sterile environments and are examined with accredited laboratories.
BASIC PACKAGE

Autologous Derived

  • Produced without genetic manipulation or cloning
  • yet the unique culture process ensures the maximum effectiveness of the therapy utilizing only your own cells.
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Evidence-based and real-world application

  • ISC therapy is safely administered in compliance with the standard health regulation.
  • No adverse effects reported but all have exhibited benefits and improvements.
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Safe and simple extraction process

  • Up to 250mL of blood is collected from the patient for treatment.
  • Culture process takes only two to four days after which reinfusion is possible.
  • The extraction process is akin to blood donations and treatment would be possible in a matter of days.
  • The fast turn-around time is virtually impossible to achieve with other stem cell treatment options.